Design and validation of ultrahigh-plex discovery panels for immuno-oncology and oncology

Ultrahigh-plex single-cell spatial phenotyping is revolutionizing cancer research through deeper interrogation of cellular and protein-level co-expression, localization, and arrangements within cancer tissues. To enable in-depth characterization of the tumor microenvironment (TME), we have developed and validated pre-optimized antibody panels on cancer tissues to reveal key cell types and cellular architecture using the PhenoCycler®-Fusion system. Here, we present our methodology for the development and validation of multiplex spatial phenotyping panels for oncology and immuno-oncology.