Single-cell spatial proteomic analysis of the tumor microenvironment in naive NSCLC samples with immunotherapy treatment and response data

The tumor microenvironment (TME) represents a complex network comprising a variety of cell types including immune, stromal, and extracellular elements in addition to malignant tumor cells. Growing evidence suggests that the spatial relationships of these components influence tumor progression and response/resistance to immunotherapeutic agents. However, the precise mechanisms driving this relationship to clinical outcomes remains poorly understood. Here, we present a workflow for analyzing spatial proteomic data of the TME at the single-cell level using the Enable Medicine platform with the aim of providing mechanistic insights into signatures of response to immunotherapy.